Human Insulin Receptor/CD220 (long isoform, His Tag) recombinant protein

Human Insulin Receptor/CD220 (long isoform, His Tag) recombinant protein

• DataSheet
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INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).

Insulin receptor

4932439J01RIK; IR; INSR; IR-B; HHF5; IR-A; D630014A15RIK; CD220

P06213

INSR

Human Cells

A DNA sequence encoding the human INSR isoform long (NP_000199.2) extracellular domain (Met 1-Lys 956) was expressed, fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human INSR isoform long consists of 940 amino acids and has a predicted molecular mass of 107 (83+24) kDa. As a result of glycosylation, the apparent molecular mass of rhINSR is approximately 125-135 kDa & 40-45 kDa, corresponding to the ? subunit and the ECD of ? subunit respectively in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. When 1 μg/ml of biotinylated human insulin is immobilized onto a streptavidin coated plate, it can bind human INSR (isoform long) with a linear range of 0.625-10 μg/ml.