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Product Name
human IgG heavy chain antibody
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Description
human IgG heavy chain Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive WB detected in human plasma tissue, Jurkat cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
IgG Fc antibody; IGHG antibody; IGHG4 antibody; Immunoglobulin heavy chain gamma antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of human IgG heavy chain recombinant protein (Accession Number: BC025985). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human plasma (0.3ug) tissue were subjected to SDS PAGE followed by western blot with Catalog No:111722(human IgG heavy chain Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111722(IGHG4 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111722(IGHG4 Antibody) at dilution of 1:50 (under 40x lens)
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Background
IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"