Human IFNGR1/CD119 (His Tag) recombinant protein

Human IFNGR1/CD119 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 ( interferon gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interteron receptor while IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-γ was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-γ exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types.

Interferon gamma receptor 1

IFGR; IMD27A; FLJ45734; RP3-503F13.3; IFNGR; IMD27B; IFN-GAMMAR; NKTAR; CD119; IFNGR1

P15260

IFNGR1

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Gly 245) of human IFN-?R1 pre-protein (NP_000407.1) was expressed, fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human IFN-?R1 consists of 239 amino acids after removal of the signal peptide and predicts a molecular mass of 27.3 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhIFN-?R1 is approximately 40-35 kDa due to the glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit rhIFN­γ mediated protection of WISH cells infected with vesicular stomatitis virus(VSV).
The ED₅₀ for this effect is typically 2-8 μg/mL.