Human ICOS Ligand/B7-H2 (His Tag) recombinant protein

Human ICOS Ligand/B7-H2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I  transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 280 amino acid precursor that contains a signal sequence, an V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.

ICOS ligand

Z39IG; RP23-23B17.3; A530061A11; BC025105; RP6-159A1.1; MGC36211; CRIG; VSIG4

O75144

ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653

Human Cells

A DNA sequence encoding the human ICOSLG (NP_056074.1) extracellular domain (Met 1-Ser 258) was fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human ICOSLG consists of 251 amino acids and has a calculated molecular mass of 28 kDa. The apparent molecular mass of the protein is approximately 50-60 kDa in SDS-PAGE under reducing conditions as predicted.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.