Human Follistatin (Fc Tag) recombinant protein

Human Follistatin (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.

Follistatin, isoform CRA_c

FS; AL033346; FST; FOLLISTATIN

P19883

FST; hCG_40892

Human Cells

A DNA sequence encoding the human FST isoform FST344 (NP_037541.1) (Met 1-Trp 344) was fused with the Fc region of human IgG1 at the C-terminus.

Human

The secreted recombinant human FST/Fc is a disulfide-linked homodimer. The reduced monomer consists of 556 amino acids and has a predicted molecular mass of 61.7 kDa. As a result of glycosylation, rh FST/Fc monomer migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

1. Measured by its ability to bind human INHBA-his in a functional ELISA.
2. Measured by its ability to bind mouse INHBA-his in a functional ELISA.
3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is typically 0.5-3 μg/mL in the presence of 10 ng/ml Recombinant Human ctivin A.