Human EPHA3/Eph Receptor A3 (His Tag) recombinant protein

Human EPHA3/Eph Receptor A3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

TYRO4; EPH RECEPTOR A3; HEK4; TYRO4 PROTEIN TYROSINE KINASE; TYROSINE-PROTEIN KINASE TYRO4; EK4; TYROSINE-PROTEIN KINASE RECEPTOR ETK1; AW492086; ETK1; HEK; ETK; MEK4; CEK4; EPHRIN TYPE-A RECEPTOR 3; END3; EPH-LIKE KINASE 4; HUMAN EMBRYO KINASE 1; EPH-LIKE TYROSINE KINASE 1; EPHA3

Human Cells

A DNA sequence encoding the human EPHA3 (Met1-Gln541) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human EPHA3 consists of 532 amino acids and predicts a molecular mass of 60.3 KDa. It migrates as an approximately 65-70 KDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc (Cat:504531), The EC₅₀ of human EphrinA5-Fc (Cat:504531) is 6.2-14.6 ng/ml.