Human DUSP3/VHR recombinant protein

Human DUSP3/VHR recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP), and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs, VHR lacks mitogen-activated protein kinase (MAPK)-binding domain, and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates, it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and, therefore, may play a role in aspects of T lymphocyte physiology that depend on these kinases.

Dual specificity protein phosphatase 3

5031436O03RIK; DUSP3; RP23-398F7.3; 2210015O03RIK; VHR; T-DSP11

P51452

DUSP3; VHR

Baculovirus-Insect Cells

A DNA sequence encoding the human VHR (P51452)(Met 1-Pro 185)was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.

Human

The secreted recombinant human VHR consists of 187 amino acids and predicts a molecular mass of 20.6 KDa. The apparent molecular mass of the protein is approximately 21 KDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by hydrolysis of 250 µM 3-O-methyl fluorescein phosphate (OMFP).
The specific activity is >1.0 µmol/min/mg.