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Product Name
Human CDK7 & CCNH & MNAT1 recombinant protein
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Uniprot ID
P50613
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
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Protein Species
Human
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Molecular weight
The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25,38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 18 nmol/min/mg using MBP as substrate.
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Validations
Human CDK7 & CCNH & MNAT1 Heterotrimer Protein SDS-PAGE
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