Human CD16a/FCGR3A (176 Val, His Tag) recombinant protein

Human CD16a/FCGR3A (176 Val, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.\CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. \

Low affinity immunoglobulin gamma Fc region receptor III-A

FCGR3A; FCGR3; FCGRIII; IGFR3; FCG3; FCRL3; CD16-2; FCRIIIA; RP11-5K23.1; CD16; FCGRIV; FCRIII; CD16A; FCGAMMARIV; FCR-10; 4833442P21RIK; IMD20

P08637

FCGR3A; CD16A; FCG3; FCGR3; IGFR3

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (AAH17865.1) was expressed, with a C-terminal polyhistidine tag. It is identical to FCGR3A158F/V in the reference.

Human

The secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD16a is approximately 48 KDa due to glycosylation.

> 97 % as determined by SDS-PAGE

1. Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80nM.
2.Measured by its binding ability in a functional ELISA.
Immobilized human CD16a-His(F158V)(Cat:503246) at 10ug/ml(100ul/well ) can bind human IgG1 with a linear range of 0.00128-0.8 μg/ml.