Human CD16a/FCGR3A (176 Phe, His & AVI Tag) recombinant protein

Human CD16a/FCGR3A (176 Phe, His & AVI Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.\CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. \

Low affinity immunoglobulin gamma Fc region receptor III-A

FCGR3A; FCGR3; FCGRIII; IGFR3; FCG3; FCRL3; CD16-2; FCRIIIA; RP11-5K23.1; CD16; FCGRIV; FCRIII; CD16A; FCGAMMARIV; FCR-10; 4833442P21RIK; IMD20

P08637

FCGR3A; CD16A; FCG3; FCGR3; IGFR3

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR3A158F/V in the reference.

Human

The secreted recombinant human CD16a consists of 223 amino acids and predicts a molecular mass of 25.1 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA.
2. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a(Cat:503735-B). The EC₅₀ of Human CD16a(Cat:503735-B) is 2.4 - 5.7 μg/ml.
3. Labeling ratio of biotin to protein: 0.7-1.