Human Bruton Tyrosine Kinase / BTK Kinase (His Tag) recombinant protein

Human Bruton Tyrosine Kinase / BTK Kinase (His Tag) recombinant protein

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Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.

XLA; AGMX1; RP1-164F3.2; BPK; MGC126261; AT; RP23-91G19.5; MGC126262; PSCTK1; AI528679; BTK; ATK; XID; IMD1

Q5JY90

Baculovirus-Insect Cells

A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.

Human

The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 KDa.

> 85 % as determined by SDS-PAGE

1. The specific activity was determined to be 115 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.
2. Measured by its ability to bind human BLNK in a functional ELISA.