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Human ACACA kinase/HMGCR kinase (G1/B2/A2) recombinant protein

Cat.#: 504677

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Product Information

  • Product Name
    Human ACACA kinase/HMGCR kinase (G1/B2/A2) recombinant protein
  • Documents
  • Protein short names
    PRKAA1; A830082D05; HAMPKB; AMPK2; AMPKA2; 2310008I11RIK; PRKAA; RP23-431F3.2; AMPK; AMPKA1; PRKAB1; AMPKALPHA2; PRKAA2
  • Uniprot ID
    P54619
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
  • Protein Species
    Human
  • Molecular weight
    The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
  • Validations

    Human AMPK (G1/B2/A2) Heterotrimer Protein SDS-PAGE

    Human AMPK (G1/B2/A2) Heterotrimer Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"