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Product Name
Human ACACA kinase/HMGCR kinase (G1/B1/A2) recombinant protein
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Protein short names
PRKAA; 2310008I11RIK; AMPKA2; AMPK2; HAMPKB; PRKAA1; A830082D05; PRKAA2; AMPKALPHA2; PRKAB1; AMPKA1; AMPK; RP23-431F3.2
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Uniprot ID
P54619
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
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Protein Species
Human
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Molecular weight
The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 40, 42 & 95 KDa respectively in SDS-PAGE under reducing conditions.
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Purity
> 94 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
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Validations
Human AMPK (G1/B1/A2) Heterotrimer Protein SDS-PAGE
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