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Product Name
HuD-specific antibody
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Description
HuD-specific Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive WB detected in mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ELAV like protein 4 antibody; ELAVL4 antibody; Hu antigen D antibody; HUD antibody; PNEM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HuD-specific recombinant protein (Accession Number: NM_001144774). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111719(ELAVL4 Antibody) at dilution of 1:2000
IP Result of anti-ELAVL4 (IP:Catalog No:111719, 4ug; Detection:Catalog No:111719 1:300) with mouse brain tissue lysate 3600ug.
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:111719(ELAVL4 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ELAVL4 represents ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
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