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Product Name
HTR3A Polyclonal Antibody
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Description
Polyclonal antibody to HTR3A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
HTR3A antibody; 5-HT-3 antibody; 5-HT3A antibody; 5-HT3R antibody; 5HT3R antibody; HTR3 antibody; 5-hydroxytryptamine receptor 3A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-241 of human HTR3A (NP_000860.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - HTR3A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HTR3A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - HTR3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using HTR3A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HTR3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using HTR3A antibody at dilution of 1:100 (40x lens).
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Background
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"