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Product Name
HSPBP1 antibody
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Description
HSPBP1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer. Positive WB detected in mouse testis tissue, human brain tissue, human heart tissue, human testis tissue. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse; other species not tested.
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Alternative names
HSPBP1 antibody; HSPBP antibody; Hsp70 binding protein 2 antibody; Hsp70 binding protein 1 antibody; Hsp70 interacting protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSPBP1 recombinant protein (Accession Number: NM_012267). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111703(HSPBP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111703(HSPBP1 antibody) at dilution of 1:100 (under 25x lens)
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Background
Hsp70-interacting protein (HSPBP1)is a protein of approximately 40 kilodaltons detected in cell extracts. Its mRNA is present in all tissues analyzed but is most abundant in heart and skeletal muscle. HSPBP1 binds the ATPase domain of Hsp70 and inhibits approximately 90% of the Hsp40-activated Hsp70 ATPase activity by preventing ATP binding to Hsp70.
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