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Product Name
HSPA4 antibody
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Description
HSPA4 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse brain tissue. Positive WB detected in L02 cells, A375 cells, HEK-293 cells, K-562 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse ovary tissue, mouse testis tissue, NIH/3T3 cells, rat brain tissue. Observed molecular weight by Western-blot: 110-120kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
APG 2 antibody; APG2 antibody; Heat shOCk 70 kDa protein 4 antibody; heat shOCk 70kDa protein 4 antibody; HS24/P52 antibody; HSP70.4 antibody; hsp70RY antibody; HSPA4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSPA4 recombinant protein (Accession Number: NM_002154). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:111698(HSPA4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111698(HSPA4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111698(HSPA4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody Catalog No:111698 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-HSPA4 (IP:Catalog No:111698, 4ug; Detection:Catalog No:111698 1:1000) with mouse brain tissue lysate 4000ug.
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Background
HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. ( 21487003)
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References
- Ullmann Y, Klein Y, Savulescu D. Salivary monitoring related to major surgery. European journal of clinical investigation. 40(12):1074-80. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"