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Product Name
HSPA2 antibody
- Documents
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Description
HSPA2 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue. Positive WB detected in NIH/3T3 cells, HeLa cells, ROS1728 cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Rat, Mouse; other species not tested.
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Alternative names
Heat shOCk 70 kDa protein 2 antibody; heat shOCk 70kDa protein 2 antibody; HSP70 2 antibody; HSP70 3 antibody; HSPA2 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of HSPA2 recombinant protein (Accession Number: NM_021979). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:107269(HSPA2 Antibody) at dilution of 1:10000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107269(HSPA2 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107269(HSPA2 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107269(HSPA2 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA4 protein in testis and multiple cell lines. (21373891)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"