-
Product Name
HSPA14 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to HSPA14
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Monkey
-
Alternative names
HSPA14 antibody; HSP70-4 antibody; HSP70L1 antibody; heat shock 70 kDa protein 14 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-509 of human HSPA14 (NP_057383.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - HSPA14 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HSPA14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - HSPA14 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using HSPA14 antibody . Blue: DAPI for nuclear staining.
-
Background
Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"