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Product Name
HSP70 antibody
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Description
HSP70 Mouse Monoclonal antibody. Positive IF detected in HEK-293 cells. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ54303 antibody; FLJ54370 antibody; FLJ54392 antibody; FLJ54408 antibody; FLJ75127 antibody; Heat shOCk 70 kDa protein 1/2 antibody; heat shOCk 70kDa protein 1A antibody; HSP70 antibody; HSP70 1 antibody; HSP70 1/HSP70 2 antibody; HSP70 1A antibody; HSP70.1/HSP70.2 antibody; HSP70I antibody; HSP72 antibody; HSPA1 antibody; HSPA1A antibody; HSPA1B antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of HSP70 recombinant protein (Accession Number: NM_005346). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:40000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107267(HSP70 Antibody) at dilution of 1:10000
Immunofluorescent analysis of (-20oc Ethanol) fixed HEK-293 cells using Catalog No:107267(HSP70 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
HSPA1A represents heat shock 70kDa protein 1A
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References
- Duan X, Wang W, Liu X, Yan H, Dai R, Lin Q. Neuroprotective effect of ethyl acetate extract from gastrodia elata against transient focal cerebral ischemia in rats induced by middle cerebral artery occlusion. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan / sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine. 35(6):671-8. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"