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Product Name
HSD3B2 Polyclonal Antibody
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Description
Polyclonal antibody to HSD3B2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
HSD3B2 antibody; HSD3B antibody; HSDB antibody; SDR11E2 antibody; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - HSD3B2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HSD3B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - HSD3B2 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using HSD3B2 antibody .
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Background
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"