-
Product Name
HSD11B1 antibody
- Documents
-
Description
HSD11B1 Rabbit Polyclonal antibody. Positive WB detected in L02 cells, Jurkat cells, mouse liver tissue. Observed molecular weight by Western-blot: 65-70 kDa
-
Tested applications
ELISA, WB
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
11 beta HSD1 antibody; 11 DH antibody; HDL antibody; HSD11 antibody; HSD11B antibody; HSD11B1 antibody; HSD11L antibody; SDR26C1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of HSD11B1 recombinant protein (Accession Number: NM_005525). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
-
Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:111542(HSD11B1 antibody) at dilution of 1:400
-
Background
HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confrming the dimeric nature of 11-beta-HSD1 in liver and In omental adipose tissue, an additional band of 50 kDa was strongly expressed(PMID:18209879).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"