-
Product Name
HRP-conjugated GST Tag antibody
- Documents
-
Description
HRP-conjugated GST Tag Mouse Monoclonal antibody. Positive WB detected in Recombinant protein. Observed molecular weight by Western-blot: -
-
Tested applications
ELISA, WB
-
Species reactivity
Tag
-
Alternative names
Glutathione S-Transferase antibody; GST tag antibody; HRP Glutathione S-Transferase antibody; HRP GST antibody
-
Isotype
Mouse IgG2a
-
Preparation
This antibody was obtained by immunization of Recombinant Protein (Accession Number: FN315687). Purification method: Protein A Purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:5000-1:50000
-
Validations
Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66001(GST Tag Antibody) at dilution of 1:10000
-
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"