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  • HRP-conjugated GFP tag antibody

HRP-conjugated GFP tag antibody

Cat.#: 107297

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Product Information

  • Product Name
    HRP-conjugated GFP tag antibody
  • Documents
  • Description
    HRP-conjugated GFP tag Mouse Monoclonal antibody. Positive WB detected in Recombinant protein. Observed molecular weight by Western-blot: -
  • Tested applications
    ELISA, WB
  • Species reactivity
    Recombinant protein, Aequorea victoria; other species not tested.
  • Alternative names
    CFP antibody; eGFP antibody; eYFP antibody; GFP antibody; GFP tag antibody; HRP CFP antibody; HRP eGFP antibody; HRP GFP antibody; HRP YFP antibody; YFP antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of Recombinant Protein (Accession Number: AVU73901). Purification method: Protein A Purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:5000-1:50000

  • Validations

    Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66002(GFP tag Antibody) at dilution of 1:10000

    Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66002(GFP tag Antibody) at dilution of 1:10000

  • Background
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"