HRP-conjugated GAPDH antibody

HRP-conjugated GAPDH antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

HRP-conjugated GAPDH Mouse Monoclonal antibody. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 36 kDa

ELISA, WB

Human, Mouse, Rat, Zebra Fish; other species not tested.

G3PD antibody; GAPD antibody; GAPDH antibody; HRP-G3PD antibody; HRP-GAPD antibody; HRP-GAPDH antibody; HRP-OK/SW cl.12 antibody; OK/SW cl.12 antibody

Mouse IgG2b

This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_001289745). Purification method: Protein A Purified.

Monoclonal

PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.

Store at -20℃. DO NOT ALIQUOT

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.