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Product Name
HRP-conjugated Flag Tag antibody
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Description
HRP-conjugated Flag Tag Mouse Monoclonal antibody. Positive WB detected in Recombinant protein. Positive IP detected in Recombinant protein. Positive IF detected in Transfected HEK-293 cells.
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DYKDDDK antibody; Flag Tag antibody; HRP conjugated Flag tag antibody; HRP-Flag tag antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Peptide. Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: N/AIF: 1:20-1:200
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Validations
Flag tagged fusion protein (20ng/lane) was subjected to SDS-PAGE followed by western blot with HRP-66008 at dilution of 1:40000.
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Background
Flag tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of Flag tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the Flag tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with Flag tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated Flag Tag antibody.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"