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Product Name
HRP-conjugated alpha Tubulin antibody
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Description
HRP-conjugated alpha Tubulin Mouse Monoclonal antibody. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 52kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alpha tubulin ubiquitous antibody; alpha tubulin antibody; a tubulin antibody; HRP Tubulin Alpha antibody; K ALPHA 1 antibody; TUBA1B antibody; tubulin antibody; Tubulin Alpha antibody; Tubulin alpha 1B chain antibody; Tubulin alpha ubiquitous chain antibody; Tubulin K alpha 1 antibody; tubulin antibody; alpha 1b antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_006082). Purification method: Protein A Purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:10000
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Validations
Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody HRP-60031 at a dilution of 1:5000.
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Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This antiody recognize Tubulin-Alpha only and it is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"