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Product Name
HRP-conjugated 6*His, His-Tag antibody
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Description
HRP-conjugated 6*His, His-Tag Mouse Monoclonal antibody. Positive WB detected in Recombinant protein. Observed molecular weight by Western-blot: -
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Tested applications
ELISA, WB
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Species reactivity
Tag
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Alternative names
6*His antibody; 6*His antibody; His Tag antibody; 6histag antibody; 6XHis antibody; HHHHHH antibody; His tag antibody; HISTIDINE TAGGED antibody; HRP 6XHis antibody; HRP HHHHHH antibody; HRP His tag antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of 6xHis Peptide. Purification method: Protein G Purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
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Validations
Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66005(6*His, His-Tag Antibody) at dilution of 1:10000
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Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"