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Product Name
HRH4 Polyclonal Antibody
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Description
Polyclonal antibody to HRH4
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
HRH4 antibody; AXOR35 antibody; BG26 antibody; GPCR105 antibody; GPRv53 antibody; H4 antibody; H4R antibody; HH4R antibody; histamine H4 receptor antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 194-304 of human HRH4 (NP_067637.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:4000
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Validations
Western blot - HRH4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HRH4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"