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Product Name
HR Polyclonal Antibody
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Description
Polyclonal antibody to HR
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
HR antibody; ALUNC antibody; AU antibody; HSA277165 antibody; HYPT4 antibody; MUHH antibody; MUHH1 antibody; lysine-specific demethylase hairless antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-480 of human HR (NP_005135.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - HR Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HR antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 40s.
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Background
Histone demethylase that specifically demethylates both mono- and dimethylated 'Lys-9' of histone H3. May act as a transcription regulator controlling hair biology (via targeting of collagens), neural activity, and cell cycle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"