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Product Name
HPD antibody
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Description
HPD Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, human liver tissue, Jurkat cells, rat liver tissue. Observed molecular weight by Western-blot: 40-45 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
4 HPPD antibody; 4HPPD antibody; GLOD3 antibody; HPD antibody; HPPDase antibody; PPD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HPD recombinant protein (Accession Number: NM_002150). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111446(HPD antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:111446(HPD antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111446(HPD antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:111446(HPD Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HPD represents 4-hydroxyphenylpyruvate dioxygenase
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