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Product Name
HOXD10 Polyclonal Antibody
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Description
Polyclonal antibody to HOXD10
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HOXD10 antibody; HOX4 antibody; HOX4D antibody; HOX4E antibody; Hox-4.4 antibody; homeobox D10 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HOXD10 (NP_002139.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - HOXD10 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HOXD10 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - HOXD10 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using HOXD10 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"