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Product Name
HOOK3 antibody
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Description
HOOK3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue. Positive IP detected in mouse kidney tissue. Positive WB detected in human liver tissue, HEK-293 cells, mouse kidney tissue. Observed molecular weight by Western-blot: 83 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ31058 antibody; h hook3 antibody; hHK3 antibody; HK3 antibody; hook homolog 3 (Drosophila) antibody; HOOK3 antibody; Protein Hook homolog 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HOOK3 recombinant protein (Accession Number: NM_032410). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111522(HOOK3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111522(HOOK3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111522(HOOK3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody Catalog No:111522 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HOOK3 (IP:Catalog No:111522, 4ug; Detection:Catalog No:111522 1:500) with mouse kidney tissue lysate 4000ug.
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Background
HOOK3 represents hook homolog 3 (Drosophila)
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