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Product Name
HOOK1 antibody
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Description
HOOK1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HEK-293 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 85 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
h hook1 antibody; hHK1 antibody; HK1 antibody; hook homolog 1 (Drosophila) antibody; HOOK1 antibody; Protein Hook homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HOOK1 recombinant protein (Accession Number: NM_015888). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111521(HOOK1 antibody) at dilution of 1:600
Immunofluorescent analysis of HEK-293 cells using Catalog No:111521(HOOK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111521(HOOK1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-HOOK1 (IP:Catalog No:111521, 4ug; Detection:Catalog No:111521 1:500) with mouse brain tissue lysate 4680ug.
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Background
HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.
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References
- Zhou J, Du YR, Qin WH. RIM-BP3 is a manchette-associated protein essential for spermiogenesis. Development (Cambridge, England). 136(3):373-82. 2009.
- Hall EA, Keighren M, Ford MJ. Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes. PLoS genetics. 9(12):e1003928. 2013.
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