-
Product Name
HNRNPUL1 antibody
- Documents
-
Description
HNRNPUL1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in A549 cells, HEK-293 cells, HeLa cells, MCF7 cells, mouse brain tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 90-115 kDa
-
Tested applications
ELISA, WB, IHC, IF, FC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
E1B AP5 antibody; E1BAP5 antibody; FLJ12944 antibody; HNRNPUL1 antibody; HNRPUL1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of HNRNPUL1 recombinant protein (Accession Number: NM_007040). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111514(HNRNPUL1 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:111514(HNRNPUL1 antibody) at dilution of 1:100 (under 25x lens)
Immunofluorescent analysis of HepG2 cells, using HNRNPUL1 antibody Catalog No:111514 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-HNRNPUL1 (IP:Catalog No:111514, 5ug; Detection:Catalog No:111514 1:2000) with HeLa cells lysate 2000ug.
1X10^6 HepG2 cells were stained with 0.2ug HNRNPUL1 antibody (Catalog No:111514, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
-
Background
HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1–Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .
-
References
- Tsai PL, Chiou NT, Kuss S, García-Sastre A, Lynch KW, Fontoura BM. Cellular RNA binding proteins NS1-BP and hnRNP K regulate influenza A virus RNA splicing. PLoS pathogens. 9(6):e1003460. 2013.
- Gurunathan G, Yu Z, Coulombe Y, Masson JY, Richard S. Arginine methylation of hnRNPUL1 regulates interaction with NBS1 and recruitment to sites of DNA damage. Scientific reports. 5:10475. 2015.
- Satterly N, Tsai PL, van Deursen J. Influenza virus targets the mRNA export machinery and the nuclear pore complex. Proceedings of the National Academy of Sciences of the United States of America. 104(6):1853-8. 2007.
- Chakraborty P, Seemann J, Mishra RK. Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. EMBO reports. 10(10):1154-60. 2009.
- Massimelli MJ, Kang JG, Majerciak V. Stability of a long noncoding viral RNA depends on a 9-nt core element at the RNA 5' end to interact with viral ORF57 and cellular PABPC1. International journal of biological sciences. 7(8):1145-60. 2011.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"