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Product Name
HNRNPM Polyclonal Antibody
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Description
Polyclonal antibody to HNRNPM
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HNRNPM antibody; CEAR antibody; HNRNPM4 antibody; HNRPM antibody; HNRPM4 antibody; HTGR1 antibody; NAGR1 antibody; hnRNP M antibody; hnRNPM antibody; heterogeneous nuclear ribonucleoprotein M antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - HNRNPM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HNRNPM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - HNRNPM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using HNRNPM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using HNRNPM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using HNRNPM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPM antibody at dilution of 1:100 (40x lens).
Immunofluorescence - HNRNPM Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using HNRNPM antibody .
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Background
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"