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Product Name
HNRNPL antibody
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Description
HNRNPL Rabbit Polyclonal antibody. Positive WB detected in RAW 264.7 cells, mouse liver tissue. Positive IHC detected in human kidney tissue, human colon cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ35509 antibody; hnRNP L antibody; HNRNPL antibody; HNRPL antibody; P/OKcl.14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HNRNPL recombinant protein (Accession Number: BC069184). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:111510(HNRNPL Antibody) at dilution of 1:600
Immunofluorescent analysis of HeLa cells using Catalog No:111510(HNRNPL Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111510(HNRNPL Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:111510(HNRNPL Antibody) at dilution of 1:50 (under 40x lens)
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Background
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing.The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.
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References
- Wang Y, Yue D, Xiao M. C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis. Journal of proteome research. 14(2):804-13. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"