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Product Name
HNRNPH2 Polyclonal Antibody
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Description
Polyclonal antibody to HNRNPH2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
HNRNPH2 antibody; FTP3 antibody; HNRPH' antibody; HNRPH2 antibody; MRXSB antibody; hnRNPH' antibody; NRPH2 antibody; heterogeneous nuclear ribonucleoprotein H2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-285 of human HNRNPH2 (NP_062543.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - HNRNPH2 Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using HNRNPH2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - HNRNPH2 Polyclonal Antibody
Immunofluorescence analysis of 293T cells using HNRNPH2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"