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Product Name
HNRNPDL Polyclonal Antibody
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Description
Polyclonal antibody to HNRNPDL
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HNRNPDL antibody; HNRNP antibody; HNRPDL antibody; JKTBP antibody; JKTBP2 antibody; LGMD1G antibody; laAUF1 antibody; heterogeneous nuclear ribonucleoprotein D-like antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 261-420 of human HNRNPDL (NP_112740.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - HNRNPDL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HNRNPDL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - HNRNPDL Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using HNRNPDL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"