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Product Name
HNRNPA1 antibody
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Description
HNRNPA1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, mouse colon tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 34kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Pig; other species not tested.
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Alternative names
Helix destabilizing protein antibody; hnRNP A1 antibody; hnRNP core protein A1 antibody; HNRNPA1 antibody; HNRPA1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HNRNPA1 recombinant protein (Accession Number: NM_002136). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111500(HNRNPA1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:111500(HNRNPA1 antibody) at dilution of 1:100 (under 10x lens)
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Background
In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.
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References
- Guo R, Li Y, Ning J, Sun D, Lin L, Liu X. HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells. PloS one. 8(4):e62729. 2013.
- Chen K, Huang C, Yuan J, Cheng H, Zhou R. Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells. Molecular biology and evolution. 31(8):2194-211. 2014.
- Markus MA, Marques FZ, Morris BJ. Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs. PloS one. 6(12):e28926. 2011.
- Lu Q, Bai J, Zhang L. Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus. Journal of proteome research. 11(5):2890-903. 2012.
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