-
Product Name
HNMT antibody
- Documents
-
Description
HNMT Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, mouse liver tissue. Positive IF detected in MCF-7 cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 33 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
histamine N methyltransferase antibody; HMT antibody; HNMT antibody; HNMT S1 antibody; HNMT S2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of HNMT recombinant protein (Accession Number: NM_006895). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111498(HNMT antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111498(HNMT antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:111498(HNMT Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
HNMT, a histamine-metabolizing enzyme, inactivates histamine by N-methylation. It plays an important role in degrading histamine and in regulating the airway response to histamine. HNMT in human astrocytes play a role in the regulation of extraneuronal histamine concentration and the activities of histaminergic neurons.
-
References
- Yoshikawa T, Naganuma F, Iida T. Molecular mechanism of histamine clearance by primary human astrocytes. Glia. 61(6):905-16. 2013.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"