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HMGN1 antibody

Cat.#: 111486

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Product Information

  • Product Name
    HMGN1 antibody
  • Documents
  • Description
    HMGN1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells. Positive IF detected in HepG2. Observed molecular weight by Western-blot: 17kd
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    human, mouse, rat; other species not tested.
  • Alternative names
    HMGN1 antibody; FLJ27265 antibody; HMG14 antibody; FLJ31471 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HMGN1 recombinant protein (Accession Number: NM_004965). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111486(HMGN1 antibody) at dilution of 1:500

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111486(HMGN1 antibody) at dilution of 1:500

    Immunofluorescent analysis of HepG2 cells, using HMGN1 antibody Catalog No:111486 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using HMGN1 antibody Catalog No:111486 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Background
    HMGN1 (high-mobility group nucleosome-binding protein 1) is a member of the HMG superfamily of nonhistone chromatin-binding proteins, which which regulate chromatin structure and gene transcription. It binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. It may participate in the process which maintains transcribable genes in an unique chromatin conformation, and inhibit the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 expressed abundantly
  • References
    • Wei F, Yang D, Tewary P. The Alarmin HMGN1 contributes to antitumor immunity and is a potent immunoadjuvant. Cancer research. 74(21):5989-98. 2014.
    • Yang D, Postnikov YV, Li Y. High-mobility group nucleosome-binding protein 1 acts as an alarmin and is critical for lipopolysaccharide-induced immune responses. The Journal of experimental medicine. 209(1):157-71. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"