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Product Name
HMGCS1 antibody
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Description
HMGCS1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in A431 cells, HepG2 cells, mouse liver tissue. Observed molecular weight by Western-blot: 57 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HMG CoA synthase antibody; HMGCS antibody; HMGCS1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HMGCS1 recombinant protein (Accession Number: NM_001330663). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:111485(HMGCS1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using HMGCS1 antibody Catalog No:111485 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HMGCS1 (IP:Catalog No:111485, 3ug; Detection:Catalog No:111485 1:1000) with mouse liver tissue lysate 4000ug.
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Background
HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).
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References
- Zhang S, Zheng L, Dong D. Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats. Food chemistry. 141(3):2108-16. 2013.
- Xu T, Zheng L, Xu L. Protective effects of dioscin against alcohol-induced liver injury. Archives of toxicology. 88(3):739-53. 2014.
- Fryer LG, Jones B, Duncan EJ. The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis. The Journal of biological chemistry. 289(7):4244-61. 2014.
- there was a problem with your PMID, try again.
- Dong D, Qi Y, Xu L. Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats. Food & function. 5(12):3065-75. 2014.
- Real CI, Megger DA, Sitek B. Identification of proteins that mediate the pro-viral functions of the interferon stimulated gene 15 in hepatitis C virus replication. Antiviral research. 100(3):654-61. 2013.
- Saraon P, Cretu D, Musrap N. Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression. Molecular & cellular proteomics : MCP. 12(6):1589-601. 2013.
- Liu M, Xu L, Yin L. Potent effects of dioscin against obesity in mice. Scientific reports. 5:7973. 2015.
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