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Product Name
HMBS antibody
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Description
HMBS Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A375 cells, HEK-293 cells, Raji cells. Positive IF detected in MCF-7 cells, Hela cells, HepG2 cells. Observed molecular weight by Western-blot: 39-42kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HMBS antibody; hydroxymethylbilane synthase antibody; PBG D antibody; PBGD antibody; Porphobilinogen deaminase antibody; Pre uroporphyrinogen synthase antibody; UPS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HMBS recombinant protein (Accession Number: NM_000190). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111473(HMBS antibody) at dilution of 1:300
Immunofluorescent analysis of MCF-7 cells, using HMBS antibody Catalog No:111473 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
HMBS represents hydroxymethylbilane synthase
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