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Product Name
HLX Polyclonal Antibody
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Description
Polyclonal antibody to HLX
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
HLX antibody; HB24 antibody; HLX1 antibody; H2.0 like homeobox antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human HLX (NP_068777.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - HLX Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HLX antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunofluorescence - HLX Polyclonal Antibody
Immunofluorescence analysis of A549 cells using HLX antibody . Blue: DAPI for nuclear staining.
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Background
Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"