-
Product Name
HLF Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to HLF
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse
-
Alternative names
HLF antibody; hepatic leukemia factor antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human HLF (NP_002117.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:1000
IF 1:50 - 1:100 -
Validations
Western blot - HLF Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HLF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - HLF Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using HLF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"