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Product Name
HLA-DOA Polyclonal Antibody
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Description
Polyclonal antibody to HLA-DOA
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Tested applications
WB
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Species reactivity
Human
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Alternative names
HLA-DOA antibody; HLA-DNA antibody; HLA-DZA antibody; HLADZ antibody; major histocompatibility complex, class II, DO alpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-135 of human HLA-DOA (NP_002110.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - HLA-DOA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HLA-DOA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"