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Product Name
HLA-DMB Polyclonal Antibody
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Description
Polyclonal antibody to HLA-DMB
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
HLA-DMB antibody; D6S221E antibody; RING7 antibody; major histocompatibility complex, class II, DM beta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-218 of human HLA-DMB (NP_002109.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - HLA-DMB Polyclonal Antibody
Western blot analysis of extracts of Raji cells, using HLA-DMB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - HLA-DMB Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using HLA-DMB antibody at dilution of 1:100 (40x lens).
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Background
Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"