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Product Name
HLA class I (HLA-C) antibody
- Documents
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Description
HLA class I (HLA-C) Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, HL-60 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 44 kDa
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Tested applications
ELISA, IHC, IP, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
D6S204 antibody; FLJ27082 antibody; HLA C antibody; HLA Cw antibody; HLA Cw12 antibody; HLA JY3 antibody; HLAC antibody; HLA-C antibody; HLC C antibody; MHC class I antigen Cw*7 antibody; PSORS1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HLA class I (HLA-C) recombinant protein (Accession Number: NM_002117). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111416(HLA class I (HLA-C) antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111416(HLA class I (HLA-C) antibody) at dilution of 1:50 (under 10x lens)
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111416(HLA class I (HLA-C) Antibody) at dilution of 1:1000
IP Result of anti-HLA class I (HLA-C) (IP:Catalog No:111416, 4ug; Detection:Catalog No:111416 1:1000) with Jurkat cells lysate 2400ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:111416(HLA class I (HLA-C) Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
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