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Product Name
HLA-A antibody
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Description
HLA-A Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human small intestine tissue. Positive WB detected in HepG2 cells, HeLa cells. Observed molecular weight by Western-blot: 41-44kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HLA A antibody; HLAA antibody; HLA-A antibody; MHC class I antigen A*3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002116). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111417(HLA-A antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111417(HLA-A Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111417(HLA-A Antibody) at dilution of 1:50 (under 40x lens)
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Background
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
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